Research grade, high sensitivity fluorescence spectrophotometer. The Hitachi F7100 features the highest sensitivity in its class and an extremely long lifetime light source as well as high speed scanning and a host of accessories.
ITEM | DESCRIPTION | |||
Sensitivity (Raman light of water) |
Noise: Background S/N 20,000 or above*6 Noise: Peak 1,200 or above*7 |
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Minimum sample volume | 0.6 mL (in use of standard 10 mm rectangular cell)*8 | |||
Photometric principle | Monochromatic light monitoring ratio calculation | |||
Light source | 150 W xenon lamp, self-deozonating lamp house | |||
Monochromator | Stigmatic
concave diffraction grating: 900 lines/mm, F2.2 Brazed wavelength: Excitation side 300 nm, emission side 400 nm |
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Measuring
wavelength range (on both EX and EM) |
200 to 750
nm, and zero-order light (Expandable up to 900 nm with optional detector) |
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Bandpass | Excitation
side: 1, 2.5, 5, 10, 20 nm Emission side: 1, 2.5, 5, 10, 20 nm |
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Resolution | 1.0 nm (at 546.1 nm) | |||
Wavelength accuracy | ±1 nm | |||
Wavelength scan speed | 30, 60, 240, 1,200, 2,400, 12,000, 30,000, 60,000 nm/min | |||
Wavelength drive speed | 60,000 nm/min | |||
3D measurement time | 3 min*9 | |||
Response | Response from 0 to 98 %:?0.002, 0.004, 0.01, 0.05, 0.1, 0.5, 2, 4 s | |||
Photometric value range | -9999 to 9999 | |||
Dimensions/weight | Spectrophotometer: 620 (W) x 520 (D) x 300 (H) mm (excluding protrusions)/41 kg | |||
Working temperature / humidity | 15 to 35°C,45 to 80% (condensation not allowed, 70% or less at 30°C or higher) | |||
Power consumption (spectrophotometer) | 100, 115, 220, 230, 240 V AC, 50/60 Hz, 380 VA | |||
FL Solutions program | Standard software | |||
Data processing unit | PC: Windows® | |||
Printer | Printer compatible with Windows® | |||
*6
EX 350 nm, Slit 10 nm, Response 4 s *7 EX 350 nm, Slit 5 nm, Response 2 s *8 Does not require cell spacer; no slit restriction *9 EX 200 to 750 nm, Sampling interval 10 nm, EM 200 to 750 nm, Sampling interval 10 nm |
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FUNCTIONS | ||||
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The F-7000 fluorescence spectrophotometer achieves a high sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min) wavelength scan at the highest level in its class, with a wide dynamic range up to 6 digits. Furthermore, it is applied to research, development, and quality control in a broad range of fields, such as industrial materials, life sciences, and biotechnology.
The 4-turret accessory used in this experiment automatically measures, by computer control, the time course of the fluorescence intensity at regular time intervals through 4 rotating cells installed in a turret. A reaction trace for an enzyme is used to measure the time course of fluorescence intensity in plural samples. Previously, the time course was measured for one sample at a time, so the measurement time required was proportional to the number of samples. Application of the automatic 4-turret accessory reduces measurement time.
An enzyme assay was applied to ethanol measurement in this experiment. The amount of ethanol in the fish is increased according to a decline of the freshness, so this measurement is referred to as the freshness index. Ethanol is usually measured with a spectrophotometer. When ethanol is present in trace amounts, a low concentration measurement is performed with a fluorescence spectrophotometer. In this experiment, the general enzyme assay reagent "F-kit ethanol (J.K.International)" was used. In addition to the enzyme assay, a spectrophotometer was used to measure ethanol amounts. The analysis conditions were optimized, and an analytical curve for a standard solution was created.
Keywords: Fluorescence spectrum, Spectrum calibration, Instrumental response, Rhodamine B, Quinine sulfate, Standard material, GLP/GMP program