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Products Fluorescence
Spectrophotometers F-7000 |
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Fluorescence Spectrophotometer
F-7000
Research grade, high sensitivity fluorescence spectrophotometer
The Hitachi F-7000 features the highest sensitivity
in its class as well as high speed scanning and a host
of accessories.
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FL33 |
ETHANOL MEASUREMENT AND
ENZYME ASSAY WITH USE OF AUTOMATIC 4-TURRET ACCESSORY |
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The F-7000 fluorescence spectrophotometer achieves a high
sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min)
wavelength scan at the highest level in its class, with
a wide dynamic range up to 6 digits. Furthermore, it is
applied to research, development, and quality control
in a broad range of fields, such as industrial materials,
life sciences, and biotechnology.
The 4-turret accessory used in this experiment automatically
measures, by computer control, the time course of the
fluorescence intensity at regular time intervals through
4 rotating cells installed in a turret. A reaction trace
for an enzyme is used to measure the time course of fluorescence
intensity in plural samples. Previously, the time course
was measured for one sample at a time, so the measurement
time required was proportional to the number of samples.
Application of the automatic 4-turret accessory reduces
measurement time.
An enzyme assay was applied to ethanol measurement in
this experiment. The amount of ethanol in the fish is
increased according to a decline of the freshness, so
this measurement is referred to as the freshness index.
Ethanol is usually measured with a spectrophotometer.
When ethanol is present in trace amounts,
a low concentration measurement is performed with a fluorescence
spectrophotometer. In this experiment, the general enzyme
assay reagent "F-kit ethanol (J.K.International)"
was used. In addition to the enzyme assay, a spectrophotometer
was used to measure ethanol amounts. The analysis conditions
were optimized, and an analytical curve for a standard
solution was created. |
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FL32 |
FLUORESCENCE SPECTRUM MEASUREMENT
METHOD ALLOWING A COMPARISON OF DIFFERENT EQUIPMENT -
SPECTRAL CALIBRATION OF INSTRUMENTAL RESPONSE AND VALIDATION
METHOD, AND MEASUREMENT EXAMPLE FOR A STANDARD FLUORESCENT
MATERIAL |
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Keywords: Fluorescence spectrum, Spectrum calibration,
Instrumental response, Rhodamine B, Quinine sulfate, Standard
material, GLP/GMP program |
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